CV
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Contact Information
| Name | Saroj Khadka |
| Professional Title | Graduate Student Researcher |
| khadka@pitt.edu | |
| Location | Pittsburgh, Pennsylvania PA 15213 |
Professional Summary
Biomedical Science PhD candidate with strong research experience in bacterial virulence mechanisms, host-pathogen interactions, and microbial gene regulation. Skilled in molecular biology and microbiological techniques, mentoring and science communication. Motivated to build an independent career in biomedical research.
Experience
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2024 - 2026 Pennsylvania, PA, USA
Graduate Student Researcher
University of Pittsburgh
- Investigate molecular mechanisms regulating Klebsiella pneumoniae capsular polysaccharide attachment and chain length.
- Develop and optimize experimental techniques for bacterial exopolysaccharide research.
- Present research findings at departmental, regional, and national scientific conferences.
- Mentor undergraduate researchers in laboratory techniques, experimental workflows, and project design.
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2020 - 2021 Biratnagar, Nepal
Molecular Biologist
B-Sure Path Lab & Diagnostic Centre
- Performed routine qRT-PCR testing for COVID-19 diagnosis from clinical specimens.
- Trained newly recruited laboratory technicians in molecular diagnostic techniques and workflows.
- Managed patient records, diagnostic reporting, and laboratory quality control in compliance with National Public Health Laboratory (Nepal) guidelines.
- Coordinated diagnostic reporting with the Ministry of Health COVID-19 Crisis Management Center.
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2018 - 2019 Kathmandu, Nepal
Secondary School Teacher
Newton Higher Secondary School
- Taught Science and Mathematics to Grade 8 students.
- Designed and administered tests, and prepared test score reports to track student progress.
- Facilitated parent-teacher meetings to discuss student development and address concerns.
- Proctored final examinations and National Education Board exams for Grade 8 and Grade 10 students.
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2018 - 2019 Kathmandu, Nepal
Intern Medical Microbiologist
Kathmandu Model Hospital
- Conducted routine clinical microbiological tests for pathogen isolation and identification in clinical specimens.
- Maintained culture collection and surveillance records of multidrug-resistant bacterial isolates for national reporting.
- Performed monthly and quarterly quality assurance assays as required by National Public Health Laboratory.
- Performed hospital environmental pathogen monitoring.
Education
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2024 - 2026 Pennsylvania, PA, USA
PhD
University of Pittsburgh
Microbiology and Immunology
- Thesis: Mechanisms and Regulation of Klebsiella pneumoniae Capsular Polysaccharide Chain Length and Attachment.
- Advisor: Dr. Laura A. Mike
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2017 - 2019 Kathmandu, Nepal
MSc
Tribhuvan University
Medical Microbiology
- Thesis: Susceptibility to Fluoroquinolones and gyrA ser83 Mutation in Salmonella enterica serovar Typhi at a Referral Hospital of Kathmandu Valley.
- Advisor: Dr. Megharaj Banjara
Awards
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2024 MMI Research Fellowship Award
Department of Medical Microbiology and Immunology, University of Toledo COLMS
Awarded by the Department of Medical Microbiology and Immunology, University of Toledo to graduate students who have received a pre-doctoral fellowship before thesis defense.
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2024 MMI Research Scholar Award
Department of Medical Microbiology and Immunology, University of Toledo COLMS
Awarded by the Department of Medical Microbiology and Immunology, University of Toledo to graduate students with a first-author research manuscript accepted or published before thesis defense.
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2023 AHA Predoctoral Fellowship
American Heart Association
Two-year fellowship awarded by the American Heart Association (AHA) in the form of stipend support to PhD students pursuing research aimed at advancing human health and well-being.
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2024 Graduate Research Award
Graduate Student Association, University of Toledo
Awarded by the Department of Medical Microbiology and Immunology, University of Toledo to graduate students with a first-author research manuscript accepted or published before thesis defense.
Publications
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2026 Sugar-Import Suppresses Klebsiella pneumoniae mucoidy in cAMP-CRP-dependent Manner
bioRxiv
This study demonstrates a new regulatory mechanism of Klebsiella pneumoniae mucoidy. Here, we show that sugars suppress mucoidy upon import which downregulates rmpADC expression and increases capsule chain length diversity, and as result suppresses mucoidy.
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2023 Urine-mediated Suppression of Hypermucoviscosity is Counteracted by Wzc Mutations
mSphere
In the second paper, he applied the quantum theory to light to explain the photoelectric effect. In particular, he used the idea of light quanta (photons) to explain experimental results, but stressed the importance of the experimental results. The importance of his work on the photoelectric effect earned him the Nobel Prize in Physics in 1921.
Skills
Languages
Certificates
- Machine Learning - Stanford University (2018)
Projects
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Systems controlling Klebsiella pneumoniae capsular polysaccharide chain length and attachment
Currently investigating the mechanisms and regulatory pathways governing Klebsiella pneumoniae capsule attachment and chain length using various molecular biology and biochemical analytical tools. The ongoing projects aim to unravel the bacterial factors and signaling pathways involved in K. pneumoniae capsular polysaccharide attachment and chain length regulation and their impact on bacterial pathogenesis.
- Advisor: Dr. Laura A. Mike
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Susceptibility to Fluoroquinolones and gyrA ser83 mutation in Salmonella enterica Serovar Typhi at a Referral Hospital of Kathmandu Valley
Conducted a hospital-based study on the prevalence of Salmonella Typhi in febrile patients visiting the Kathmandu Model Hospital and evaluated resistance to fluoroquinolones associated with gyrA ser83 mutation in Salmonella Typhi and Paratyphi isolated from the patients.
- Advisor: Dr. Megharaj Banjara
References
- Dr. Laura A. Mike
Assistant Professor, University of Pittsburgh, USA. Email: laura.mike@pitt.edu
- Dr. Megharaj Banjara
Associate Professor, Tribhuvan University, Nepal. Email: banjaramr@gmail.com